The Dual Synergy of Photodynamic and Sonodynamic Therapy in the Eradication of Methicillin-Resistant Staphylococcus aureus

Recently, the combined application of synergistic therapies for photodynamic antimicrobial chemotherapy has become important to obtain more efficient results. The synergism between two sensitizers, rose bengal (RB) and chlorin e6 (Ce6), excited by different methods, was evaluated as a novel approach both sonodynamic therapy against methicillin-resistant Staphylococcus aureus. sonostability singlet oxygen generation (with 1,3-diphenylisobenzofuran RB tetrathiafulvalene Ce6) were measured under sonication (1 MHz, 3 W) using spectrophotometer. Ce6 remained stable during sonication. sonosensitizer than Ce6. dual noticed, achieving >3 log reduction molar ratios RB:Ce6 1:1 1:3, while, alone, sensitizers with ultrasound light, respectively, achieved only ca. 1 reduction.